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At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader

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Used needles and syringes should be placed in an FDA-cleared puncture-proof sharps container rather than household trash to prevent needle-stick injuries

Research Context & Applications Research involving BPC-157 often focuses on how signaling pathways influence communication between different tissue environments, including muscular , connective , gastrointestinal , and epithelial systems